Journal: Acta neuropathologica
Article Title: TREM2 expression in the brain and biological fluids in prion diseases.
doi: 10.1007/s00401-021-02296-1
Figure Lengend Snippet: Fig. 3 mRNA Trem2 expression in the brain of sCJD MM1 and VV2 mouse models. RT-qPCR analysis Trem2 expression in mouse models of sCJD MM1 (tg340-CJD MM1) (left panels) and sCJD VV2 (tg361-CJD VV2) (right panels) subtypes. Trem2 expression was analysed in five brain regions: anterior and posterior cortex, cer- ebellum, olfactory bulb, and brainstem, at three time points: pre-clinical (80 days post inoculation (dpi)), early-clinical (120 dpi), and clinical (180 dpi). CJD-inoculated animals (black columns) were compared to their corresponding control- inoculated animals (white columns). Four to eight animals per time point and condition were used. Normalization was performed using the house- keeping gene Hprt; *p < 0.05, **p < 0.01
Article Snippet: Human TREM2 goat polyclonal antibody (RD Systems, AF1828) was used diluted 1:20 for immunohistochemistry and at a dilution of 1:200 for western blotting.
Techniques: Expressing, Quantitative RT-PCR, Control